Mcp 1 is a member of the cytokine family and plays an important role in immunoregulation and inflammatory processes. Preparation of mcp 1 standard dilutions general elisa protocol 1. Biolegends elisa max deluxe set is a sandwich enzymelinked immunosorbent assay elisa. This elisa reagent set is specifically engineered for accurate and precise measurement of human. Mcp 1 chemoattractant protein involed in monocyte migration and infiltration monocyte chemotractant protein 1 mcp 1, also known as ccl2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. Prepare all reagents, samples and standards as instructed in the manual. Rat mcp 1 elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant mcp 1 in a sandwich elisa format. The following is a protocol that was developed for the tgf. Human mcp1monocyte chemotactic protein 1 elisa kit. Incubate 2 hours at room temperature or 4 c overnight. The biotin mouse antihuman mcp1 should be titrated 0. Mcp 1 monocyte chemotactic protein 1, ccl2 has a predicted molecular weight of approximately 11kda, however, it may run at a higher molecular weight due to glycosylation.
Enzymelinked immunosorbent assay elisa mcp1 levels in eps and in cell culture supernatants were measured by a human ccl2 mcp1 elisa readysetgo kit. Aspirate and wash 7 times with 30 sec to 1 min soaks. Standards and samples are added to the wells, and mcp 1 binds to the immobilized capture antibody. Kt020 product the kassay human mcp1 elisa is an enzymelinked immunosorbent. A human mcp 1 specific mouse monoclonal antibody is first coated on a 96well plate.
Dilute the antigen to 12 ugml in coating solution 2. The enzyme linked immunosorbent assay elisa is a powerful method for detecting and quantifying a specific protein in a complex mixture. Mcp1 is believed to be identical to je, a gene whose expression is induced in mouse fibroblasts by plateletderived growth factor cochran and others 1983. Mcp1 is produced by a variety of cell types, either. Rat mcp1 elisa development kit leinco technologies. The mouse monocyte chemoattractant protein1ccl2 mcp1 uncoated elisa kit contains prematched antibody pairs, plates and reagents for performing quantitative. Rat mcp1 elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant rat mcp1 in a sandwich elisa format within the range of 162000 pgml. The omnikine murine jemcp1 elisa kit contains the components necessary for quanititative determination of natureal or recombinant murine jemcp1 concentrations within any. Bgk500 elisa kit for the accurate quantitation of human mcp1 ccl2 concentrations in cell culture supernates. Biotin mouse antihuman mcp1 can be paired with purified mouse antihuman mcp1 clone 10f7.
The monocyte chemoattractant protein1 mcp 1ccl2 is a member of the cc chemokine family, and a potent chemotactic factor for monocytes. Determine the number of microwell strips required to test the desired number of samples. Standards and samples are added to the wells, and mcp1 binds to the immobilized capture antibody. Mouse mcp1 ccl2 elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 9. Page 1 of 1 mouse ccl2 mcp1 uncoated elisa catalog number. Mcp1ccl2 human uncoated elisa kit with plates invitrogen. Boster is proud to announce the newest picokine fast elisa kits. Wash buffer concentrate 20x item b 25 ml of 20x concentrated solution. The human ccl2 mcp1 uncoated elisa reagent set with or without highaffinity binding microwell plates contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays elisa. A human mcp1 specific mouse monoclonal antibody is first coated on a 96well. Immunogen recombinant rat mcp1 application for research use only. Mcp1 elisa kit mouse monocyte chemoattractant protein. Human mcp1 ccl2 elisa kit ab179886 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of mcp1 protein in human serum, plasma, urine, and. The human mcp1 ccl2 elisa kit accurately measures natural human mcp1 ccl2 levels quantified versus standard curves obtained and is.
Originally described by engvall and perlmann 1971, the method enables analysis of protein samples immobilized in microplate wells using specific antibodies. Urinary monocyte chemoattractant protein1 mcp1 is a. The sandwich elisa protocol provided here is representative of most readytouse elisa kits for measurement of cytokines, chemokines, growth factors, and other extracellular targets. The loss rate of human mcp1monocyte chemotactic protein 1 elisa kit is less than 5% within the expiration date under appropriate storage condition. It is a member of the ccbeta family of cytokines, characterized by the cysc. Monocyte chemotactic protein1 mcp1ccl2 is associated. Product name rat mcp 1 elisa kit catalog number elrmcp1 kit components component size description mcp 1 microplate item a 96 wells 12 strips x 8 wells coated with antirat mcp 1. Mcp subfamily composed of at least four members mcp 1, 2, 3, and 4. Mcp1 monocyte chemotactic protein1, ccl2 has a predicted molecular weight of approximately 11kda, however, it may run at a higher molecular weight due to glycosylation.
The mouse monocyte chemoattractant protein 1 ccl2 mcp 1 uncoated elisa kit contains prematched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays elisa to detect and quantify protein levels of mouse mcp 1. Human mcp 1 is composed of 76 amino acids and is kda in size. The human monocyte chemoattractant protein 1 ccl2 mcp 1 uncoated elisa kit contains prematched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays elisa to detect and quantify protein levels of human mcp 1. Your affordable premium quality elisa kits provider. The human ccl2 mcp1 uncoated elisa reagent set with or without highaffinity binding microwell plates contains the necessary reagents, buffers and diluents for performing. With automated equipment the assay is easily scalable for set up in highthroughput and can handle a large number of samples in parallel, making it a popular. This mcp1ccl2 human elisa kit from aviscera bioscience enables to detect mcp1 on human samples. Rat mcp1 elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant rat mcp1 in a sandwich elisa format within the. Preparation of mcp1 standard dilutions general elisa protocol 1. Product name rat mcp1 elisa kit catalog number elrmcp1 kit components component size description mcp1 microplate item a 96 wells 12 strips x 8 wells coated with antirat mcp. The rat mcp1 elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of rat mcp1 in cell lysate and tissue. A 384well simplestep elisa microplate is available to use as an alternative to the 96well microplate provided with simplestep elisa kits.
Mcp1 elisa kits invitrogen mcp1ccl2 mouse uncoated elisa kit with plates print share invitrogen mcp1ccl2 mouse uncoated elisa kit with plates. Rat mcp1 ccl2 elisa kit for cell and tissue lysates. Mcp 1 is mainly expressed by macrophages in response to a wide range of cytokines such as il6, tnfalpha and il1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells. Invitrogen mcp1ccl2 mouse uncoated elisa kit with plates specifically engineered for accurate and precise measurement of mouse ccl2 protein levels from samples including serum, plasma, and cell culture supernatants. Sek104 to achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the general elisa protocol. Protocol i elisa for tracking disease outbreaks step 1.
Track disease transmission protocol ii antigen detection elisa pregnancy, drug, gmo, antigen. Sek104 to achieve the best assay results, this manual must be read carefully before using this product and the assay is run as. Mcp1 elisa kit mouse monocyte chemoattractant protein 1. A human mcp1 specific mouse monoclonal antibody is first coated on a 96well plate. Human mcp1 is composed of 76 amino acids and is kda in size. Mcp1 is mainly expressed by macrophages in response to a. The kit is stable for a period of up to 3 months from the date of receipt. Add 100 ul of diluted antigen to appropriate wells. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency. The below protocol is a sample protocol for mouse mcp1 ccl2 elisa kit using a biotinylated detection antibody and streptavidinhrp. Classroom exchange hiv, sars and west nile antigen of simulated bodily fluids viruses, common cold, step 2. Kassay kamiya biomedical companya biomedical company rev. Human mcp1monocyte chemotactic protein 1 elisa kittk06435.
Elisa intra assay consistency is measured using wells from the same plateassay kit. Mcp1 measurements were performed as per the manufacturers recommendations. This immunoassay has been shown to accurately quantitate recombinant human mcp 1. Cytokines and chemokines and related molecules, energy metabolism and body weight regulation.
Mcp1 elisa kit human monocyte chemoattractant protein. Kt020 product the kassay human mcp1 elisa is an enzymelinked immunosorbent assay for the quantitative determination of mcp1 in human cell culture supernatant, serum, plasma and amniotic fluid. The loss rate of human mcp 1 monocyte chemotactic protein 1 elisa kit is less than 5% within the expiration date under appropriate storage condition. As of march 6, 2018, human mcp1 elisa kit has been redeveloped. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. Urine and serum samples were centrifuged at 2500 r. Invitrogen mcp1ccl2 mouse uncoated elisa kit with plates. Sandwich elisas allow for the detection and quantification of an analyte in a sample by using known analyte concentrations as standards and plotting absorbance of known concentrations vs known standard. Bgk500 elisa kit for the accurate quantitation of human mcp1 ccl2 concentrations in cell culture supernates, serum, plasma, and other biological fluids.
Sets provide all the timesavings, convenience and performance of a highend, commercial elisa kit for a fraction of the price. Mcp1ccl2 mouse uncoated elisa kit with plates invitrogen. Human ccl2 mcp1 uncoated elisa enzymelinked immunosorbent assay for quantitative detection of human ccl2 mcp1 catalog number 887399 pub. Mouse mcp1 elisa kit picokine sandwich high sensitivity elisa kit for quantitative detection of mouse mcp1. Mcp1 elisa kit human monocyte chemoattractant protein 1. Elisa inter assay consistency is measured using wells from different plates from the same batch productionlot. Please read the datasheet in its entirety before using this product. As of march 6, 2018, human mcp 1 elisa kit has been redeveloped. Mcp 1 is produced by a variety of cell types, either constitutively or after induction by oxidative stress, cytokines, or growth factors.
Invitrogen mcp1ccl2 mouse uncoated elisa kit with plates 10. Depending on the protein of interest, antibodies, buffers, or substrates being used, this general protocol may need to be optimized. Notes mcp1 ccl2 is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory stimuli. Notes mcp 1 ccl2 is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory. The enzymelinked immunosorbent assay elisa is a frequently used application which determines the concentration of an analyte in solution. We measured random samples of human mcp1 ccl2 elisa kit picokine within the same batchlot to ensure the consistency of the kits performances. Mcp1was characterized as a monocytespecific chemoattractant that was later shown to attract also t lymphocytes and nk cells. Immunoreagents is the direct us manufacturer of quality antibodies and reagents used in research and in vitro diagnostics. Apr 01, 2010 enzymelinked immunosorbent assay elisa mcp1 levels in eps and in cell culture supernatants were measured by a human ccl2 mcp1 elisa readysetgo kit ebioscience, san diego, ca. Highly sensitive 96well immunoassay kit with additional reagents.
Human mcp1 ccl2 precoated elisa kit catalog number. Jemcp1 murine omnikine elisa kit assaybiotechnology. Elisa technical guide and protocols thermo fisher scientific. Mouse mcp1 elisa max standard elisa development systems engineered for sensitive, precise and reliable serum and plasma cytokine quantitationbiolegends elisa max sets. Not for further distribution without written consent. The rat mcp1 elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of rat mcp1 in cell lysate and tissue lysate. Using the elisa protocol described below, this kit provides sufficient reagents to assay mcp 1 in approximately 1500 elisa plate wells.
Human mcp1 ccl2 elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 9. Mcp subfamily composed of at least four members mcp1, 2, 3, and 4. Rat mcp1 elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant mcp1 in a sandwich elisa format. The omnikine murine je mcp 1 elisa kit contains the components necessary for quanititative determination of natureal or recombinant murine je mcp 1 concentrations within any experimental sample including cell lysates, serum and plasma. Antigen detection cholera, smallpox, anthrax, elisa protocol influenza, and stds step 3. Raybio human mcp1 ccl2 elisa kit for cell culture supernatants, plasma, and serum samples. Prepare all reagents and working standards as directed in the previous sections. Mcp 1was characterized as a monocytespecific chemoattractant that was later shown to attract also t lymphocytes and nk cells.
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